Open AccessIntravenous Administration of Endothelial Colony‐Forming Cells Overexpressing Integrin β 1 Augments Angiogenesis in Ischemic Legs
Author(s) -
Goto Kazuko,
Takemura Genzou,
Takahashi Tomoyuki,
Okada Hideshi,
Kanamori Hiromitsu,
Kawamura Itta,
Watanabe Takatomo,
Morishita Kentaro,
Tsujimoto Akiko,
Miyazaki Nagisa,
Ushikoshi Hiroaki,
Kawasaki Masanori,
Mikami Atsushi,
Kosai Ken-ichiro,
Minatoguchi Shinya
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0096
Subject(s) - angiogenesis , integrin , extracellular matrix , fibronectin , progenitor cell , microbiology and biotechnology , therapeutic angiogenesis , cell therapy , immunology , chemistry , biology , neovascularization , stem cell , cancer research , cell , biochemistry
The reparative capacity of endothelial progenitor cells appears to be limited by their poor survival when injected directly into ischemic tissue. Human endothelial colony‐forming cells (ECFCs) were transduced using a lentiviral vector encoding integrin β 1 (ITGB1) or enhanced green fluorescent protein. Blood perfusion of the ischemic limb was significantly augmented only in the ITGB1‐ECFC group. Intravenous administration of ECFCs engineered to home to ischemic tissue appears to efficiently mediate therapeutic angiogenesis.