Open AccessTargeted Disruption of the β 2 ‐Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells
Author(s) -
Wang Dachun,
Quan Yuan,
Yan Qing,
Morales John E.,
Wetsel Rick A.
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0049
Subject(s) - biology , embryonic stem cell , beta 2 microglobulin , cd8 , human leukocyte antigen , cytotoxic t cell , stem cell , microbiology and biotechnology , immune system , immunology , antigen , gene , genetics , in vitro
It was hypothesized that transplanting human embryonic stem cells (hESCs) lacking human leukocyte antigen class I (HLA‐I) expression would not result in a robust immune rejection by allogeneic recipients. β 2 ‐Microglobulin‐null hESCs transplanted into natural killer cell‐depleted immunocompetent mice developed tumors resembling those from control hESCs in severe combined immunodeficiency mice. β 2 ‐Microglobulin‐null hESCs significantly reduced immunogenicity to CD8 + T cells and might provide a renewable source of cells for tissue regeneration without HLA matching.