Open AccessStructural Changes in N ‐Glycans on Induced Pluripotent Stem Cells Differentiating Toward Cardiomyocytes
Author(s) -
Kawamura Takuji,
Miyagawa Shigeru,
Fukushima Satsuki,
Kashiyama Noriyuki,
Kawamura Ai,
Ito Emiko,
Saito Atsuhiro,
Maeda Akira,
Eguchi Hiroshi,
Toda Koichi,
Miyagawa Shuji,
Okuyama Hiroomi,
Sawa Yoshiki
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0029
Subject(s) - induced pluripotent stem cell , glycan , fucose , fucosylation , chemistry , cellular differentiation , microbiology and biotechnology , in vitro , biochemistry , biology , embryonic stem cell , gene , galactose , glycoprotein
N ‐Glycans were isolated from human induced pluripotent stem cells (iPSCs), iPSC‐derived cardiomyocytes (iPSC‐CMs), and human cardiomyocytes (hCMCs), and analyzed. The exposed N ‐acetylglucosamine (GlcNAc) and the nonreduced terminal fucose types of N ‐glycans decreased, whereas the exposed galactose or the α2‐3 NeuAc types increased in the iPSCs during cardiomyogenic differentiation. More bisecting GlcNAc and the triantennary structures were found in the iPSC‐CMs than hCMCs or iPSCs, and MGAT3 expression was higher in iPSCs and iPSC‐CMs than in hCMCs.