Open AccessTumor‐Free Transplantation of Patient‐Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration
Author(s) -
El Khatib Moustafa M.,
Ohmine Seiga,
Jacobus Egon J.,
Tonne Jason M.,
Morsy Salma G.,
Holditch Sara J.,
Schreiber Claire A.,
Uetsuka Koji,
Fusaki Noemi,
Wigle Dennis A.,
Terzic Andre,
Kudva Yogish C.,
Ikeda Yasuhiro
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0017
Subject(s) - induced pluripotent stem cell , reprogramming , biology , transplantation , cancer research , microbiology and biotechnology , regenerative medicine , stem cell , embryonic stem cell , cell , genetics , medicine , gene
Transplantation of progenitors from induced pluripotent stem cells reprogrammed by lentiviral vectors led to the formation of invasive teratocarcinoma‐like tumors in more than 90% of immunodeficient mice. Combined transgene‐free reprogramming and elimination of residual pluripotent cells by enzymatic dissociation ensured tumor‐free transplantation, ultimately enabling regeneration of type 1 diabetes‐specific human islet structures in vivo.