Open AccessComparison of Different Cytokine Conditions Reveals Resveratrol as a New Molecule for Ex Vivo Cultivation of Cord Blood‐Derived Hematopoietic Stem Cells
Author(s) -
Heinz Niels,
Ehrnström Birgitta,
Schambach Axel,
Schwarzer Adrian,
Modlich Ute,
Schiedlmeier Bernhard
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0284
Subject(s) - ex vivo , cord blood , stem cell , stem cell factor , haematopoiesis , biology , transplantation , thrombopoietin , hematopoietic stem cell , microbiology and biotechnology , cytokine , resveratrol , immunology , cancer research , in vivo , pharmacology , medicine
The potential of different cord blood‐derived hematopoietic stem cell (CB‐HSC) expansion strategies was compared by phenotypical analysis in vitro. Resveratrol was identified as a new, alternative small molecule that combined with cytokines to facilitate serum‐free ex vivo expansion of human CB‐HSCs. Cultivation in resveratrol preserved the CB‐HSC phenotype in vitro most efficiently and was ∼2 times more potent than commonly used cytokine conditions and the recently established serum‐free culture.