Open AccessProductive Infection of Human Embryonic Stem Cell‐Derived NKX2.1 + Respiratory Progenitors With Human Rhinovirus
Author(s) -
Jenny Robert A.,
Hirst Claire,
Lim Sue Mei,
Goulburn Adam L.,
Micallef Suzanne J.,
Labonne Tanya,
Kicic Anthony,
Ling Kak-Ming,
Stick Stephen M.,
Ng Elizabeth S.,
Trounson Alan,
Giudice Antonietta,
Elefanty Andrew G.,
Stanley Edouard G.
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0274
Subject(s) - rhinovirus , biology , endoderm , embryonic stem cell , stem cell , induced pluripotent stem cell , progenitor cell , respiratory epithelium , immunology , cellular differentiation , microbiology and biotechnology , epithelium , virus , genetics , gene
This study describes the development of a serum‐free protocol for the generation of NKX2.1 + endoderm that, when transplanted into immunodeficient mice, matured into respiratory cell types identified by expression of CC10, MUC5AC, and surfactant proteins. The experiments provide proof of principle for the use of pluripotent stem cell‐derived respiratory epithelial cells in the study of cell‐virus interactions, demonstrating that human respiratory progenitor cells derived from stem cells can be productively infected with human rhinovirus.