Open AccessA Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder‐Free and Xeno‐Free Culture Conditions: A Clinically Compliant Approach
Author(s) -
Chou Bin-Kuan,
Gu Haihui,
Gao Yongxing,
Dowey Sarah N.,
Wang Ying,
Shi Jun,
Li Yanxin,
Ye Zhaohui,
Cheng Tao,
Cheng Linzhao
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0214
Subject(s) - induced pluripotent stem cell , reprogramming , biology , cell culture , microbiology and biotechnology , cord blood , stem cell , nucleofection , regenerative medicine , cell therapy , peripheral blood mononuclear cell , computational biology , embryonic stem cell , cell , immunology , transfection , genetics , gene , in vitro
A method for highly efficient human induced pluripotent stem (iPS) cell derivation from adult blood under clinically compliant conditions is reported. The revised episomal vectors and the removal of animal‐sourced materials resulted in feeder‐free and xeno‐free iPS cell generation. Pooled cultures were purified by the presence of the TRA‐1‐60 pluripotency surface antigen to reduce clonal variations and for repaid iPS cell expansion. These new improvements permit to generate clinically compliant iPS cell lines for therapeutic applications.