Open AccessPolarized Human Embryonic Stem Cell‐Derived Retinal Pigment Epithelial Cell Monolayers Have Higher Resistance to Oxidative Stress‐Induced Cell Death Than Nonpolarized Cultures
Author(s) -
Hsiung Jamie,
Zhu Danhong,
Hinton David R.
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0205
Subject(s) - embryonic stem cell , oxidative stress , microbiology and biotechnology , programmed cell death , cell , stem cell , pigment , cell culture , retinal , retinal pigment epithelium , biology , apoptosis , chemistry , genetics , botany , biochemistry , gene , organic chemistry
To gain an understanding of how transplanted retinal pigment epithelium (RPE) cells will respond to the highly oxidatively stressed environment of an age‐related macular degeneration (AMD) patient eye, this study compared the survival of polarized and nonpolarized human embryonic stem cell (hESC)‐derived RPE cultures following oxidative stress treatment. The results suggest that implantation of polarized monolayers of hESC‐RPE cells for treating AMD patients with geographic atrophy should have better survival than injections of hESC‐RPE cells in suspension.