Open AccessOcular Surface Reconstruction With a Tissue‐Engineered Nasal Mucosal Epithelial Cell Sheet for the Treatment of Severe Ocular Surface Diseases
Author(s) -
Kobayashi Masakazu,
Nakamura Takahiro,
Yasuda Makoto,
Hata Yuiko,
Okura Shoki,
Iwamoto Miyu,
Nagata Maho,
Fullwood Nigel J.,
Koizumi Noriko,
Hisa Yasuo,
Kinoshita Shigeru
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0169
Subject(s) - pathology , transplantation , ex vivo , progenitor cell , basement membrane , goblet cell , stem cell , tissue engineering , conjunctiva , medicine , epithelium , biology , microbiology and biotechnology , in vivo , biomedical engineering , surgery
The feasibility of using an autologous tissue‐engineered cultivated nasal mucosal epithelial cell sheet (CNMES) for ocular surface reconstruction was investigated using a novel technique for the culture of nasal mucosal epithelial cells expanded ex vivo from biopsy‐derived human nasal mucosal tissues. The CNMESs were transplanted onto the ocular surfaces of rabbits and the survival of this tissue, including the goblet cells, was confirmed up to 2 weeks.