Open AccessDevelopment of a Universal RNA Beacon for Exogenous Gene Detection
Author(s) -
Guo Yuanjian,
Lu Zhongju,
Cohen Ira Stephen,
Scarlata Suzanne
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0166
Subject(s) - molecular beacon , rna , transfection , cell , population , biology , gene , gene expression , stem cell , microbiology and biotechnology , computational biology , genetics , medicine , environmental health , oligonucleotide
Stem cell therapy requires a nontoxic and high‐throughput method to achieve a pure cell population to prevent teratomas that can occur if even one cell in the implant has not been transformed. A promising method to detect and separate cells expressing a particular gene is RNA beacon technology. However, developing a successful, specific beacon to a particular transfected gene can take months to develop and in some cases is impossible. Here, we report on an off‐the‐shelf universal beacon that decreases the time and cost of applying beacon technology to select any living cell population transfected with an exogenous gene.