Open AccessReliable Generation of Induced Pluripotent Stem Cells From Human Lymphoblastoid Cell Lines
Author(s) -
Barrett Robert,
Ornelas Loren,
Yeager Nicole,
Mandefro Berhan,
Sahabian Anais,
Lenaeus Lindsay,
Targan Stephan R.,
Svendsen Clive N.,
Sareen Dhruv
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0121
Subject(s) - induced pluripotent stem cell , reprogramming , regenerative medicine , biology , germ layer , microbiology and biotechnology , spinal muscular atrophy , stem cell , embryonic stem cell , cell , genetics , gene
This study reports a new and more reliable method for lymphoblastoid cell line (LCL) reprogramming using episomal plasmids expressing pluripotency factors and p53 shRNA in combination with small molecules. This method paves the way for using invaluable worldwide LCL repositories to generate new human iPSC lines, thus providing an enormous bioresource for disease modeling, drug discovery, and regenerative medicine applications.