Open AccessDerivation and Long‐Term Culture of Transgene‐Free Human Induced Pluripotent Stem Cells on Synthetic Substrates
Author(s) -
Villa-Diaz Luis Gerardo,
Kim Jin Koo,
Lahann Joerg,
Krebsbach Paul H.
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0087
Subject(s) - induced pluripotent stem cell , transgene , regenerative medicine , in vitro , microbiology and biotechnology , in vivo , cell culture , ammonium hydroxide , embryonic stem cell , biology , stem cell , chemistry , biochemistry , gene , genetics , organic chemistry
This study describes a platform to derive, culture, and differentiate genomically stable, transgene‐free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel. Three iPSC lines derived in these conditions demonstrated continuous self‐renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of continuous culture, showing the strength this alternative platform offers to generate and maintain human iPSCs for regenerative medicine.