Derivation and Long‐Term Culture of Transgene‐Free Human Induced Pluripotent Stem Cells on Synthetic Substrates | Zendy

Villa-Diaz Luis Gerardo | Zendy; Kim Jin Koo | Zendy; Lahann Joerg | Zendy; Krebsbach Paul H. | Zendy

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Derivation and Long‐Term Culture of Transgene‐Free Human Induced Pluripotent Stem Cells on Synthetic Substrates

Author(s) -

Villa-Diaz Luis Gerardo,

Kim Jin Koo,

Lahann Joerg,

Krebsbach Paul H.

Publication year - 2014

Publication title -

stem cells translational medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.781

H-Index - 71

eISSN - 2157-6580

pISSN - 2157-6564

DOI - 10.5966/sctm.2014-0087

Subject(s) - induced pluripotent stem cell , stem cell , transgene , biology , term (time) , microbiology and biotechnology , embryonic stem cell , computational biology , genetics , physics , gene , quantum mechanics

This study describes a platform to derive, culture, and differentiate genomically stable, transgene‐free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel. Three iPSC lines derived in these conditions demonstrated continuous self‐renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of continuous culture, showing the strength this alternative platform offers to generate and maintain human iPSCs for regenerative medicine.

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