A Massive Suspension Culture System With Metabolic Purification for Human Pluripotent Stem Cell‐Derived Cardiomyocytes | Zendy

Hemmi Natsuko | Zendy; Tohyama Shugo | Zendy; Nakajima Kazuaki | Zendy; Kanazawa Hideaki | Zendy; Suzuki Tomoyuki | Zendy; Hattori Fumiyuki | Zendy; Seki Tomohisa | Zendy; Kishino Yoshikazu | Zendy; Hirano Akinori | Zendy; Okada Marina | Zendy; Tabei Ryota | Zendy; Ohno Rei | Zendy; Fujita Chihana | Zendy; Haruna Tomoko | Zendy; Yuasa Shinsuke | Zendy; Sano Motoaki | Zendy; Fujita Jun | Zendy; Fukuda Keiichi | Zendy

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A Massive Suspension Culture System With Metabolic Purification for Human Pluripotent Stem Cell‐Derived Cardiomyocytes

Author(s) -

Hemmi Natsuko,

Tohyama Shugo,

Nakajima Kazuaki,

Kanazawa Hideaki,

Suzuki Tomoyuki,

Hattori Fumiyuki,

Seki Tomohisa,

Kishino Yoshikazu,

Hirano Akinori,

Okada Marina,

Tabei Ryota,

Ohno Rei,

Fujita Chihana,

Haruna Tomoko,

Yuasa Shinsuke,

Sano Motoaki,

Fujita Jun,

Fukuda Keiichi

Publication year - 2014

Publication title -

stem cells translational medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.781

H-Index - 71

eISSN - 2157-6580

pISSN - 2157-6564

DOI - 10.5966/sctm.2014-0072

Subject(s) - induced pluripotent stem cell , embryoid body , mesenchymal stem cell , biology , stem cell , embryonic stem cell , regenerative medicine , microbiology and biotechnology , kosr , biochemistry , gene

Pluripotent markers such as Oct3/4 and Tra‐1‐60 were expressed in embryoid bodies 2 weeks after differentiation in the massive suspension culture system. Metabolic purification of cardiomyocytes (CMs) in glucose‐depleted and lactate‐enriched medium successfully eliminated residual undifferentiated stem cells, resulting in a refined CM population. Purified CMs never induced teratomas, and enriched CMs showed proper electrophysiological properties and calcium transients.

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