Open AccessEfficient and Cost‐Effective Generation of Mature Neurons From Human Induced Pluripotent Stem Cells
Author(s) -
Badja Cherif,
Maleeva Galyna,
El-Yazidi Claire,
Barruet Emilie,
Lasserre Ma,
Tropel Philippe,
Binetruy Bernard,
Bregestovski Piotr,
Magdinier Frédérique
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0024
Subject(s) - induced pluripotent stem cell , embryoid body , biology , directed differentiation , neuroscience , cellular differentiation , microbiology and biotechnology , embryonic stem cell , progenitor cell , stem cell , somatic cell , neuron , biochemistry , gene
The authors describe a feeder‐free method of generating induced pluripotent stem cells by relying on the use of a chemically defined medium that overcomes the need for embryoid body formation and neuronal rosette isolation for neuronal precursors and terminally differentiated neuron production. This specific and efficient single‐step strategy allows the production of mature neurons in 20–40 days with multiple applications, especially for modeling human pathologies.