Open AccessRemotely Activated Mechanotransduction via Magnetic Nanoparticles Promotes Mineralization Synergistically With Bone Morphogenetic Protein 2: Applications for Injectable Cell Therapy
Author(s) -
Henstock James R.,
Rotherham Michael,
Rashidi Hassan,
Shakesheff Kevin M.,
El Haj Alicia J.
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0017
Subject(s) - mechanotransduction , microbiology and biotechnology , mesenchymal stem cell , chemistry , bone morphogenetic protein 2 , biomedical engineering , biophysics , materials science , in vitro , biochemistry , biology , medicine
In this investigation, functionalized magnetic nanoparticles were attached to either the mechanically gated TREK1 K + channel or the (integrin) RGD‐binding domains of human mesenchymal stem cells. In both models, the combination of mechanical stimulation and sustained release of bone morphogenetic protein 2 (BMP2) from polymer microspheres showed a significant additive effect on mineralization, increasing the effectiveness of BMP2 delivery and demonstrating that nanoparticle‐mediated mechanotransduction can be used synergistically with pharmacological approaches for orthopedic tissue engineering to maximize bone formation.