Open AccessSelection Based on FOXA2 Expression Is Not Sufficient to Enrich for Dopamine Neurons From Human Pluripotent Stem Cells
Author(s) -
Aguila Julio Cesar,
Blak Alexandra,
van Arensbergen Joris,
Sousa Amaia,
Vázquez Nerea,
Aduriz Ariane,
Gayosso Mayela,
Lopez Mato Maria Paz,
Lopez de Maturana Rakel,
Hedlund Eva,
Sonntag Kai-Christian,
Sanchez-Pernaute Rosario
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0011
Subject(s) - induced pluripotent stem cell , foxa2 , biology , embryonic stem cell , microbiology and biotechnology , stem cell , progenitor cell , cellular differentiation , neural stem cell , dopamine , neuroscience , genetics , gene
A selection approach is described that uses lentivirus vectors to express green fluorescent protein under the promoter region of FOXA2, which gives rise to dopamine neurons during embryogenesis. Our results suggest that FOXA2 is not sufficient to induce a dopaminergic fate in this system but that a combined approach of promoter activation and lentivirus vector technology can be used to select a defined cell population from a variety of human pluripotent stem cell lines.