Open AccessCapillary Force Seeding of Hydrogels for Adipose‐Derived Stem Cell Delivery in Wounds
Author(s) -
Garg Ravi K.,
Rennert Robert C.,
Duscher Dominik,
Sorkin Michael,
Kosaraju Revanth,
Auerbach Lauren J.,
Len James,
Chung Michael T.,
Paik Kevin,
Nimpf Johannes,
Rajadas Jayakumar,
Longaker Michael T.,
Gurtner Geoffrey C.
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0007
Subject(s) - self healing hydrogels , mesenchymal stem cell , stem cell , biomedical engineering , tissue engineering , wound healing , regeneration (biology) , angiogenesis , pullulan , microbiology and biotechnology , cell therapy , adipose tissue , chemistry , progenitor cell , biology , immunology , medicine , cancer research , biochemistry , polysaccharide , organic chemistry
To optimize hydrogel cell seeding, a capillary force‐based approach was developed and compared with previously established cell seeding methods. Adipose‐derived mesenchymal stem cell (ASC) viability and functionality following capillary hydrogel seeding were analyzed. Capillary seeding of ASCs within a pullulan‐collagen hydrogel bioscaffold provides a convenient and simple way to deliver therapeutic cells to wound environments. Moreover, ASC‐seeded constructs display a significant potential to accelerate wound healing that can be easily translated to a clinical setting.