Open AccessA Defined, Controlled Culture System for Primary Bovine Chromaffin Progenitors Reveals Novel Biomarkers and Modulators
Author(s) -
Masjkur Jimmy,
Levenfus Ian,
Lange Sven,
Arps-Forker Carina,
Poser Steve,
Qin Nan,
Vukicevic Vladimir,
Chavakis Triantafyllos,
Eisenhofer Graeme,
Bornstein Stefan R.,
Ehrhart-Bornstein Monika,
Androutsellis-Theotokis Andreas
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0211
Subject(s) - chromaffin cell , adrenal medulla , progenitor cell , biology , fetal bovine serum , microbiology and biotechnology , basic fibroblast growth factor , cell culture , dopaminergic , neural stem cell , stem cell , endocrinology , medicine , growth factor , catecholamine , dopamine , biochemistry , receptor , genetics
A defined, serum‐free culture system of putative adrenomedullary chromaffin progenitor cells can be used for target identification in drug discovery programs. Cholera toxin increases dopamine content relative to total catecholamines and combined treatment with Angiopoietin2, Delta4, and JAK Inhibitor increases cell number. The transcription factor Hes3, a downstream mediator of the combined treatment, is a new putative biomarker of adrenomedullary chromaffin progenitors.