Open AccessRemoval of Reprogramming Transgenes Improves the Tissue Reconstitution Potential of Keratinocytes Generated From Human Induced Pluripotent Stem Cells
Author(s) -
Igawa Ken,
Kokubu Chikara,
Yusa Kosuke,
Horie Kyoji,
Yoshimura Yasuhide,
Yamauchi Kaori,
Suemori Hirofumi,
Yokozeki Hiroo,
Toyoda Masashi,
Kiyokawa Nobutaka,
Okita Hajime,
Miyagawa Yoshitaka,
Akutsu Hidenori,
Umezawa Akihiro,
Katayama Ichiro,
Takeda Junji
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0179
Subject(s) - reprogramming , transgene , induced pluripotent stem cell , biology , microbiology and biotechnology , stem cell , cell culture , genetics , embryonic stem cell , gene
Morphological, functional, and molecular analyses including single‐cell gene expression profiling revealed that keratinocytes from transgene‐free human induced pluripotent stem cell (hiPSC) lines were more similar to normal human keratinocytes than those from transgene‐residual hiPSC lines, which may be partly explained by reactivation of residual transgenes upon induction of keratinocyte differentiation. These results suggest that transgene‐free hiPSC lines should be chosen for therapeutic purposes.