Open AccessMessenger RNA‐ Versus Retrovirus‐Based Induced Pluripotent Stem Cell Reprogramming Strategies: Analysis of Genomic Integrity
Author(s) -
Steichen Clara,
Luce Eléanor,
Maluenda Jérôme,
Tosca Lucie,
Moreno-Gimeno Inmaculada,
Desterke Christophe,
Dianat Noushin,
Goulinet-Mainot Sylvie,
Awan-Toor Sarah,
Burks Deborah,
Marie Joëlle,
Weber Anne,
Tachdjian Gérard,
Melki Judith,
Dubart-Kupperschmitt Anne
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0158
Subject(s) - reprogramming , induced pluripotent stem cell , retrovirus , biology , stem cell , computational biology , microbiology and biotechnology , genetics , cell , embryonic stem cell , gene
Differentiation studies indicated that mRNA‐derived induced pluripotent stem cells (iPSCs) differentiated efficiently into hepatoblasts and that these cells did not load additional copy number variations during differentiation. The integration‐free hepatoblasts that were generated constitute a new tool for the study of diseased hepatocytes derived from patients' iPSCs and their use in the context of stem cell‐derived hepatocyte transplantation.