Open AccessDirected Differentiation of Human Induced Pluripotent Stem Cells Toward Bone and Cartilage: In Vitro Versus In Vivo Assays
Author(s) -
Phillips Matthew D.,
Kuznetsov Sergei A.,
Cherman Natasha,
Park Kyeyoon,
Chen Kevin G.,
McClendon Britney N.,
Hamilton Rebecca S.,
McKay Ronald D.G.,
Chenoweth Josh G.,
Mallon Barbara S.,
Robey Pamela G.
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0154
Subject(s) - induced pluripotent stem cell , microbiology and biotechnology , mesenchymal stem cell , stem cell , cellular differentiation , biology , in vivo , embryonic stem cell , cartilage , bone marrow , stromal cell , in vitro , clinical uses of mesenchymal stem cells , progenitor cell , adult stem cell , immunology , cancer research , anatomy , endothelial stem cell , biochemistry , gene
The ability to generate large numbers of bone‐ and cartilage‐forming cells from induced pluripotent stem cells (iPSCs) would mark a major advance in tissue engineering. A number of protocols exist, but the overall quality and consistency of this type of differentiation are under‐reported. In this study, the authors analyzed differentiated iPSCs in vitro and in vivo by stringent criteria, and found that in vitro analysis does not predict in vivo differentiation.