Open AccessDerivation of Myogenic Progenitors Directly From Human Pluripotent Stem Cells Using a Sphere‐Based Culture
Author(s) -
Hosoyama Tohru,
McGivern Jered V.,
Van Dyke Jonathan M.,
Ebert Allison D.,
Suzuki Masatoshi
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0143
Subject(s) - induced pluripotent stem cell , myogenesis , progenitor cell , microbiology and biotechnology , biology , stem cell , embryonic stem cell , myocyte , cellular differentiation , directed differentiation , immunology , genetics , gene
The authors present a novel protocol for deriving myogenic progenitors from human embryonic stem cells and induced pluripotent stem cells using free‐floating spherical culture. Results show that sphere‐based cultures of human pluripotent stem cells, expanded in medium containing high concentrations of fibroblast growth factor and epidermal growth factor, can propagate myogenic progenitors from human embryonic stem cells and healthy and disease‐specific induced pluripotent stem cells.