Open AccessEfficient and Rapid Derivation of Primitive Neural Stem Cells and Generation of Brain Subtype Neurons From Human Pluripotent Stem Cells
Author(s) -
Yan Yiping,
Shin Soojung,
Jha Balendu Shekhar,
Liu Qiuyue,
Sheng Jianting,
Li Fuhai,
Zhan Ming,
Davis Janine,
Bharti Kapil,
Zeng Xianmin,
Rao Mahendra,
Malik Nasir,
Vemuri Mohan C.
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0080
Subject(s) - neural stem cell , induced pluripotent stem cell , human induced pluripotent stem cells , stem cell , biology , neuroscience , microbiology and biotechnology , embryonic stem cell , genetics , gene
This study developed a highly efficient serum‐free pluripotent stem cell (PSC) neural induction medium that can induce human PSCs into primitive neural stem cells (NSCs) in 7 days, obviating the need for time‐consuming, laborious embryoid body generation or rosette picking. This method of primitive NSC derivation sets the stage for the scalable production of clinically relevant neural cells for cell therapy applications in good manufacturing practice conditions.