Open AccessProspectively Isolated NGN3‐Expressing Progenitors From Human Embryonic Stem Cells Give Rise to Pancreatic Endocrine Cells
Author(s) -
Cai Qing,
Bonfanti Paola,
Sambathkumar Rangarajan,
Vanuytsel Kim,
Vanhove Jolien,
Gysemans Conny,
Debiec-Rychter Maria,
Raitano Susanna,
Heimberg Harry,
Ordovas Laura,
Verfaillie Catherine M.
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0078
Subject(s) - progenitor cell , biology , pdx1 , enteroendocrine cell , embryonic stem cell , pancreas , stem cell , microbiology and biotechnology , ductal cells , pax4 , reprogramming , endocrine system , cellular differentiation , medicine , endocrinology , hormone , cell , islet , insulin , genetics , gene , transcription factor , homeobox
This study investigated whether it is possible to isolate pancreatic endocrine progenitors from differentiating human embryonic stem cell (hESC) cultures by lineage tracing of NGN3 . Results indicate that NGN3 + cells represent pancreatic endocrine progenitors in humans. This hESC reporter line is a unique tool that may aid in gaining insight into the developmental mechanisms underlying fate choices in human pancreas and in developing cell‐based therapies.