Open AccessGeneration of Functional Insulin‐Producing Cells From Mouse Embryonic Stem Cells Through 804G Cell‐Derived Extracellular Matrix and Protein Transduction of Transcription Factors
Author(s) -
Kaitsuka Taku,
Noguchi Hirofumi,
Shiraki Nobuaki,
Kubo Takuya,
Wei Fan-Yan,
Hakim Farzana,
Kume Shoen,
Tomizawa Kazuhito
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0075
Subject(s) - embryonic stem cell , microbiology and biotechnology , stem cell , extracellular matrix , biology , transcription factor , signal transduction , genetics , gene
This study tried to establish an effective method of generating pancreatic β cells. Transduction of three transcription factors (Pdx1, NeuroD, and MafA) important to pancreatic β‐cell development induced the differentiation of mouse embryonic stem and mouse induced pluripotent stem cells into insulin‐producing cells. Results suggest the direct delivery of recombinant proteins and treatment with laminin‐5‐rich extracellular matrix to be useful for the generation of insulin‐producing cells.