Open AccessZinc Finger Nuclease‐Expressing Baculoviral Vectors Mediate Targeted Genome Integration of Reprogramming Factor Genes to Facilitate the Generation of Human Induced Pluripotent Stem Cells
Author(s) -
Phang Rui-Zhe,
Tay Felix Chang,
Goh Sal-Lee,
Lau Cia-Hin,
Zhu Haibao,
Tan Wee-Kiat,
Liang Qingle,
Chen Can,
Du Shouhui,
Li Zhendong,
Tay Johan Chin-Kang,
Wu Chunxiao,
Zeng Jieming,
Fan Weimin,
Toh Han Chong,
Wang Shu
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0043
Subject(s) - reprogramming , induced pluripotent stem cell , zinc finger nuclease , biology , sox2 , klf4 , transcription activator like effector nuclease , genetics , transgene , gene , microbiology and biotechnology , zinc finger , genome editing , transcription factor , embryonic stem cell , genome
This study reports the successful reprogramming of human fibroblasts into a state of pluripotency by baculoviral transduction‐mediated, site‐specific integration of OKSM transcription factor genes into the AAVS1 locus in human chromosome 19. Methods based on site‐specific integration of reprogramming factor genes as reported here hold the potential for efficient generation of genetically amenable induced pluripotent stem cells suitable for future gene therapy applications.