Open AccessRapid and Efficient Directed Differentiation of Human Pluripotent Stem Cells Into Retinal Pigmented Epithelium
Author(s) -
Buchholz David E.,
Pennington Britney O.,
Croze Roxanne H.,
Hinman Cassidy R.,
Coffey Peter J.,
Clegg Dennis O.
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0163
Subject(s) - induced pluripotent stem cell , embryonic stem cell , stem cell , transplantation , retinal pigment epithelium , biology , microbiology and biotechnology , macular degeneration , directed differentiation , retinal disorder , cellular differentiation , retina , medicine , neuroscience , ophthalmology , genetics , gene
Although protocols to generate retinal pigmented epithelium (RPE) from human pluripotent stem cells have become more efficient since the first report in 2004, they are still time‐consuming and relatively inefficient. This work describes an experiment wherein the addition of defined factors at specific times led to conversion of approximately 80% of the cells to an RPE phenotype in only 14 days. This protocol should be useful for rapidly generating RPE for transplantation as well as for studying RPE development in vitro.