Open AccessIdentification and Isolation of Small CD44‐Negative Mesenchymal Stem/Progenitor Cells From Human Bone Marrow Using Elutriation and Polychromatic Flow Cytometry
Author(s) -
Hall Sean R.R.,
Jiang Yajuan,
Leary Elizabeth,
Yavanian Greg,
Eminli Sarah,
O'Neill David W.,
Marasco Wayne A.
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0155
Subject(s) - cd90 , mesenchymal stem cell , cd44 , population , stem cell , progenitor cell , bone marrow , biology , flow cytometry , stromal cell , immunology , microbiology and biotechnology , cd34 , cancer research , medicine , cell , biochemistry , environmental health
This study used polychromatic flow cytometry and counterflow centrifugal elutriation to identify a phenotypically distinct population of mesenchymal stem/progenitor cells (MSPCs) within human bone marrow (BM). These BM‐MSPCs are rare, small CD45 − CD73 + CD90 + CD105 + cells that lack expression of CD44 and elute early with other adult stem cells; contrary to the widely held view that MSCs are large cells, these MSPCs are absent in later elutriation fractions that contain larger cells such as monocytes. In addition, these MSPCs give rise to mesenchymal stem/stromal cells (MSCs) that have phenotypic and functional properties that are distinct from those of BM‐MSCs purified by plastic adherence.