Open AccessA Novel Approach to Collecting Satellite Cells From Adult Skeletal Muscles on the Basis of Their Stress Tolerance
Author(s) -
Shigemoto Taeko,
Kuroda Yasumasa,
Wakao Shohei,
Dezawa Mari
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0130
Subject(s) - stem cell , myod , microbiology and biotechnology , skeletal muscle , biology , stem cell marker , myocyte , p19 cell , flow cytometry , regeneration (biology) , adult stem cell , cellular differentiation , myogenesis , anatomy , genetics , gene
This study developed a novel method of adult stem cell collection. Long‐term trypsin incubation (LTT), which led to a remarkable increase in the ratio of Pax7(+) cells that retain normal myogenic stem cell function, is proposed as a novel approach to collecting satellite cells from mouse and human skeletal muscles. This approach, based on the stress resistance of adult stem cells, is a safe and inexpensive method of efficiently collecting human satellite cells and may also be used for collecting other tissue stem cells whose surface marker is unknown.