A Novel Approach to Collecting Satellite Cells From Adult Skeletal Muscles on the Basis of Their Stress Tolerance | Zendy

Shigemoto Taeko | Zendy; Kuroda Yasumasa | Zendy; Wakao Shohei | Zendy; Dezawa Mari | Zendy

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A Novel Approach to Collecting Satellite Cells From Adult Skeletal Muscles on the Basis of Their Stress Tolerance

Author(s) -

Shigemoto Taeko,

Kuroda Yasumasa,

Wakao Shohei,

Dezawa Mari

Publication year - 2013

Publication title -

stem cells translational medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.781

H-Index - 71

eISSN - 2157-6580

pISSN - 2157-6564

DOI - 10.5966/sctm.2012-0130

Subject(s) - stem cell , myod , microbiology and biotechnology , skeletal muscle , biology , stem cell marker , myocyte , p19 cell , flow cytometry , regeneration (biology) , adult stem cell , cellular differentiation , myogenesis , anatomy , genetics , gene

This study developed a novel method of adult stem cell collection. Long‐term trypsin incubation (LTT), which led to a remarkable increase in the ratio of Pax7(+) cells that retain normal myogenic stem cell function, is proposed as a novel approach to collecting satellite cells from mouse and human skeletal muscles. This approach, based on the stress resistance of adult stem cells, is a safe and inexpensive method of efficiently collecting human satellite cells and may also be used for collecting other tissue stem cells whose surface marker is unknown.

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