Open AccessA Simple and Scalable Process for the Differentiation of Retinal Pigment Epithelium From Human Pluripotent Stem Cells
Author(s) -
Maruotti Julien,
Wahlin Karl,
Gorrell David,
Bhutto Imran,
Lutty Gerard,
Zack Donald J.
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0106
Subject(s) - induced pluripotent stem cell , microbiology and biotechnology , macular degeneration , biology , retinal pigment epithelium , cell culture , stem cell , cell type , mesenchymal stem cell , cell , retina , embryonic stem cell , medicine , neuroscience , genetics , ophthalmology , gene
This study describes a simple and scalable protocol for the generation of retinal pigment epithelial (RPE) cells from human pluripotent stem cells (hPSCs) that is less labor‐intensive than published protocols to date. After amplification by clonal propagation using a myosin inhibitor, differentiation was induced in monolayers of hPSCs, and the resulting RPE cells were purified by two rounds of whole‐dish single‐cell passage. This approach yielded highly pure populations of functional hPSC‐derived RPE cells that display many characteristics of native RPE, including pigmentation and characteristic morphology, marker expression, polarized membrane and vascular endothelial growth factor secretion, and phagocytic activity.