Derivation of Multiple Cranial Tissues and Isolation of Lens Epithelium‐Like Cells From Human Embryonic Stem Cells | Zendy

Mengarelli Isabella | Zendy; Barberi Tiziano | Zendy

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Derivation of Multiple Cranial Tissues and Isolation of Lens Epithelium‐Like Cells From Human Embryonic Stem Cells

Author(s) -

Mengarelli Isabella,

Barberi Tiziano

Publication year - 2013

Publication title -

stem cells translational medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.781

H-Index - 71

eISSN - 2157-6580

pISSN - 2157-6564

DOI - 10.5966/sctm.2012-0100

Subject(s) - embryonic stem cell , microbiology and biotechnology , biology , ectoderm , cranial neural crest , cd15 , neural stem cell , stem cell , neural crest , anatomy , embryogenesis , embryo , genetics , gene , cd34

Differentiation of human embryonic stem cells was induced in conditions that allowed formation of neural and non‐neural ectoderm and to a lesser extent mesoderm. A combination of surface markers was found that allows the isolation by fluorescence‐activated cell sorting of multiple cell populations with lens forming ability, and c‐Met/HGFR was identified as a principal marker for early detection of lens epithelium.

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