Open AccessDerivation of Multiple Cranial Tissues and Isolation of Lens Epithelium‐Like Cells From Human Embryonic Stem Cells
Author(s) -
Mengarelli Isabella,
Barberi Tiziano
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0100
Subject(s) - embryonic stem cell , microbiology and biotechnology , biology , ectoderm , cranial neural crest , cd15 , neural stem cell , stem cell , neural crest , anatomy , embryogenesis , embryo , genetics , gene , cd34
Differentiation of human embryonic stem cells was induced in conditions that allowed formation of neural and non‐neural ectoderm and to a lesser extent mesoderm. A combination of surface markers was found that allows the isolation by fluorescence‐activated cell sorting of multiple cell populations with lens forming ability, and c‐Met/HGFR was identified as a principal marker for early detection of lens epithelium.