Human Mesenchymal Stromal Cells: Identifying Assays to Predict Potency for Therapeutic Selection | Zendy

Deskins Desirae L. | Zendy; Bastakoty Dikshya | Zendy; Saraswati Sarika | Zendy; Shinar Andrew | Zendy; Holt Ginger E. | Zendy; Young Pampee P. | Zendy

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Human Mesenchymal Stromal Cells: Identifying Assays to Predict Potency for Therapeutic Selection

Author(s) -

Deskins Desirae L.,

Bastakoty Dikshya,

Saraswati Sarika,

Shinar Andrew,

Holt Ginger E.,

Young Pampee P.

Publication year - 2013

Publication title -

stem cells translational medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.781

H-Index - 71

eISSN - 2157-6580

pISSN - 2157-6564

DOI - 10.5966/sctm.2012-0099

Subject(s) - mesenchymal stem cell , in vivo , stromal cell , cell therapy , in vitro , biology , in vitro toxicology , multipotent stem cell , cell culture , bone marrow , granulation tissue , cellular differentiation , wound healing , stem cell , progenitor cell , cancer research , immunology , microbiology and biotechnology , gene , biochemistry , genetics

The goal of this study was to identify feasible and reproducible in vitro assays to predict mesenchymal stromal cell (MSC) potency. MSC lines were generated from normal human bone marrow samples and characterized by their growth, proliferation, and viability as determined by cell count, bromodeoxyuridine incorporation, and cellular ATP levels, respectively. It was found that high performance in a combination of in vitro tests accurately predicted which lines functioned well in vivo, suggesting that reliable and reproducible in vitro assays may be used to measure the functional potential of MSCs for therapeutic use.

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