Open AccessAdaptation to Robust Monolayer Expansion Produces Human Pluripotent Stem Cells With Improved Viability
Author(s) -
Kunova Michaela,
Matulka Kamil,
Eiselleova Livia,
Salykin Anton,
Kubikova Iva,
Kyrylenko Sergiy,
Hampl Ales,
Dvorak Petr
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0081
Subject(s) - induced pluripotent stem cell , adaptation (eye) , stem cell , human induced pluripotent stem cells , microbiology and biotechnology , biology , computational biology , neuroscience , embryonic stem cell , genetics , gene
This study presents an efficient feeder‐free, high‐density monolayer system in which human pluripotent stem cells (hPSCs) become SSEA‐3‐high and gradually more viable than their feeder‐dependent counterparts without changes attributed to culture adaptation. This monolayer culture system is highly suitable for long‐term, large‐scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell‐based therapies.