Open AccessDifferentiation of CD133 + Stem Cells From Amyotrophic Lateral Sclerosis Patients Into Preneuron Cells
Author(s) -
González-Garza Maria Teresa,
Martínez Héctor R.,
Caro-Osorio Enrique,
Cruz-Vega Delia E.,
Hernández-Torre Martin,
Moreno-Cuevas Jorge E.
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0077
Subject(s) - stem cell , nestin , glial fibrillary acidic protein , neurosphere , biology , cd90 , adult stem cell , neural stem cell , stem cell marker , microbiology and biotechnology , amyotrophic lateral sclerosis , homeobox protein nanog , cellular differentiation , induced pluripotent stem cell , pathology , immunology , embryonic stem cell , medicine , immunohistochemistry , cd34 , biochemistry , gene , disease
The objective of this work was to evaluate the in vitro capacity of CD133 + stem cells from 13 amyotrophic lateral sclerosis (ALS) patients to differentiate into neuron lineages. Stem cells were cultured in a control medium or a neuroinduction medium for 2–48 hours, and the expression of neuronal genes was analyzed. It was found that CD133 + stem cells from ALS patients, like the stem cells of healthy subjects, are capable of differentiating into preneuron cells.