Open AccessUse of a Synthetic Xeno‐Free Culture Substrate for Induced Pluripotent Stem Cell Induction and Retinal Differentiation
Author(s) -
Tucker Budd A.,
Anfinson Kristin R.,
Mullins Robert F.,
Stone Edwin M.,
Young Michael J.
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0040
Subject(s) - induced pluripotent stem cell , microbiology and biotechnology , biology , stem cell , cell culture , chemistry , genetics , embryonic stem cell , gene
The purpose of this study was to determine whether a proprietary xeno‐free synthetic culture surface could be used to aid in the production and subsequent retinal‐specific differentiation of clinical‐grade induced pluripotent stem cells (iPSCs). It was found that Synthemax cell culture surfaces provide an ideal surface for the xeno‐free production, culture, and differentiation of adult somatic cell‐derived iPSCs. These findings demonstrate the potential utility of these surfaces for the production of clinical‐grade retinal neurons for transplantation and induction of retinal regeneration.