Open AccessCell Therapy with Human Renal Cell Cultures Containing Erythropoietin‐Positive Cells Improves Chronic Kidney Injury
Author(s) -
Yamaleyeva Liliya M.,
Guimaraes-Souza Nadia K.,
Krane Louis S.,
Agcaoili Sigrid,
Gyabaah Kenneth,
Atala Anthony,
Aboushwareb Tamer,
Yoo James J.
Publication year - 2012
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2011-0048
Subject(s) - renal cortex , kidney , medicine , erythropoietin , acute kidney injury , fibrosis , kidney disease , endocrinology
The aims of this study were (a) to determine whether human renal cultures (human primary kidney cells [hPKC]) can be enriched in erythropoietin‐positive cells (hPKC(F+)) by using magnetic‐bead sorting; (b) to characterize the hPKC(F+) following cell separation; and (c) to establish that intrarenal delivery of enriched hPKC(F+) would be more beneficial in the treatment of renal injury, inflammation, and oxidative stress than unsorted hPKC cultures in a chronic kidney injury model. The results indicate that hPKC(F+) may be used as components of cell‐based therapies for degenerative kidney diseases.