Open AccessMagnetic Resonance Imaging and Fluorescence Labeling of Clinical‐Grade Mesenchymal Stem Cells Without Impacting Their Phenotype: Study in a Rat Model of Stroke
Author(s) -
Detante Olivier,
Valable Samuel,
de Fraipont Florence,
Grillon Emmanuelle,
Barbier Emmanuel Luc,
Moisan Anaïck,
Arnaud Josiane,
Moriscot Christine,
Segebarth Christoph,
Hommel Marc,
Remy Chantal,
Richard Marie-Jeanne
Publication year - 2012
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2011-0043
Subject(s) - mesenchymal stem cell , in vivo , magnetic resonance imaging , transplantation , stem cell , pathology , viability assay , ex vivo , cell , stroke (engine) , medicine , biomedical engineering , microbiology and biotechnology , chemistry , biology , surgery , radiology , biochemistry , mechanical engineering , engineering
Human mesenchymal stem cells (hMSCs) have strong potential for cell therapy after stroke. Tracking stem cells in vivo following a graft can provide insight into many issues regarding optimal route and/or dosing. hMSCs were labeled for magnetic resonance imaging (MRI) and histology with micrometer‐sized superparamagnetic iron oxides (M‐SPIOs) that contained a fluorophore. We assessed whether M‐SPIO labeling obtained without the use of a transfection agent induced any cell damage in clinical‐grade hMSCs and whether it may be useful for in vivo MRI studies after stroke. M‐SPIOs provided efficient intracellular hMSC labeling and did not modify cell viability, phenotype, or in vitro differentiation capacity. Following grafting in a rat model of stroke, labeled hMSCs could be detected using both in vivo MRI and fluorescent microscopy until 4 weeks following transplantation. However, whereas good label stability and unaffected hMSC viability were observed in vitro, grafted hMSCs may die and release iron particles in vivo.