Open AccessBiochemical and physiological analysis in carrot seeds from different orders of umbels
Author(s) -
Patrícia Pereira da Silva,
Marcelo Coelho Sekita,
Denise Cunha Fernandes dos Santos Dias,
Warley Marcos Nascimento
Publication year - 2016
Publication title -
ciência agronômica/revista ciência agronômica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.424
H-Index - 26
eISSN - 1806-6690
pISSN - 0045-6888
DOI - 10.5935/1806-6690.20160049
Subject(s) - umbel , germination , peroxidase , apx , horticulture , superoxide dismutase , catalase , biology , botany , chemistry , biochemistry , enzyme , cultivar
In carrot seed production, harvesting of the umbels is generally staggered, or sometimes the whole plant is harvested with the umbels at different stages of maturation; in that case, there is a risk of harvesting immature seeds and of losses due to natural dehiscent, or to physiological deterioration as a result of unfavourable environmental factors. The present study aimed to evaluate the physiological and biochemical changes related to the maturation of 'Planalto' carrot seeds from diferent umbels. The samples were collected 120 days after anthesis, with the primary, secondary and tertiary umbels being harvested separately. Seeds were analysed for germination, first count and germination rate, at temperatures of 25 ºC (optimum) to 35 ºC (adverse), as well as for emergence in the greenhouse and the emergence rate. Analyses were also carried out of the enzymes peroxidase (POX), ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD), and of the total protein. Seeds obtained from the primary and secondary umbels displayed a higher rate of germination than from the tertiary umbels, with the seeds from the primary umbels showing high activity for peroxidase, catalase and superoxide dismutase, and low activity for the enzyme ascorbate peroxidase