Open AccessCorrelation between sperm DNA fragmentation index and semen parameters in 418 men seen at a fertility center
Author(s) -
Láyonal Germán Acosta Campos,
Lissett Chiscul Requejo,
Carlos Antonio Rivas Miñano,
Jheny Díaz Orrego,
Elmer Chávez Loyaga,
Luis Gonzales Cornejo
Publication year - 2021
Publication title -
jbra
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 14
eISSN - 1518-0557
pISSN - 1517-5693
DOI - 10.5935/1518-0557.20200079
Subject(s) - dna fragmentation , semen , andrology , fertility , sperm , gynecology , medicine , semen analysis , biology , infertility , apoptosis , genetics , pregnancy , population , environmental health , programmed cell death
Objective: The aim of this study was to determine the correlation between semen parameters and sperm DNA fragmentation index (DFI) in 418 men who attended a fertility center. Methods: This retrospective study includes 418 male patients seen at In Vitro Gestar, Chiclayo - Perú between October 2013 and March 2020. DFI was determined between normozoospermic patients (NORMO) and patients with one altered parameter (ALTERED). The patients were classified as oligozoospermic [Severe/Moderate oligozoospermia (OLIGO SM): <5x106 sperm/mL and Mild oligozoospermia (OLIGO M): 5 to <15x106 sperm/mL], asthenozoospermic [Severe asthenozoospermia (ASTHENO S): <10% PM (progressive motility); Moderate asthenozoospermia (ASTHENO MO): 10 to <20% PM; Mild asthenozoospermia (ASTHENO M): 20 to <32% PM)], teratozoospermic (TERATO) and oligoasthenoteratozoospermic (OAT). DFI was determined between these groups and NORMO. The data was analyzed using the SPSS 22.0 software for Windows. Results: Normozoospermic patients presented lower significant levels of DFI compared with all groups. NORMO (15.64±7.65) vs [ALTERED (18.41±9.43, p =0.003); OLIGO SM (26.38±12.94, p <0.005); ASTHENO S (23.09±11.45, p <0.01); TERATO (17.96±9.23, p <0.05) and OAT (22.05±12.15, p =0.001)]. We determined a significant negative correlation between DFI and progressive motility (r= -0.162 p =0.001), and those with normal morphology (r= -0.100 p =0.040). Likewise the DFI shows a significant positive correlation with age (r= 0.257 p =0.000). Conclusions: Our study establishes that high DFI is accompanied by a significant impairment in all seminal parameters.