Open AccessNew approaches regarding the in vitro maturation of oocytes: manipulating cyclic nucleotides and their partners in crime
Author(s) -
Ramon César Botigelli,
E. M. Razza,
Elisa Mariano Pioltine,
Marcelo Fábio Gouveia Nogueira
Publication year - 2017
Publication title -
jbra
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 14
eISSN - 1518-0557
pISSN - 1517-5693
DOI - 10.5935/1518-0557.20170010
Subject(s) - oocyte , embryo , biology , context (archaeology) , in vitro maturation , human fertilization , microbiology and biotechnology , in vitro fertilisation , embryo quality , andrology , genetics , medicine , paleontology
Several discoveries have been described recently (5-10 years) about the biology of ovarian follicles (oocyte, cumulus cells and granulosa cells), including new aspects of cellular communication, the control of oocyte maturation and the acquisition of oocyte competence for fertilization and further embryo development. These advances are nourishing assisted reproduction techniques (ART) with new possibilities, in which novel culture systems are being developed and tested to improve embryo yield and quality. This mini-review aims to describe how the recent knowledge on the physiological aspects of mammalian oocyte is reflecting as original or revisited approaches into the context of embryo production. These new insights include recent findings on the mechanisms that control oocyte maturation, especially modulating intraoocyte levels of cyclic nucleotides during in vitro maturation using endogenous or exogenous agents. In this mini-review we also discuss the positive and negative effects of these manipulations on the outcoming embryo.