Open AccessTriplex Real-time Polymerase Chain Reaction Optimization for AZF Y-chromosome Microdeletion Analysis
Author(s) -
Tatiana Puga Torres,
Xavier Blum Rojas,
Medardo Blum Narváez,
Edith López Montanero,
Alexandra Narváez Sarasti
Publication year - 2015
Publication title -
jbra
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 14
eISSN - 1518-0557
pISSN - 1517-5693
DOI - 10.5935/1518-0557.20150011
Subject(s) - testis determining factor , sequence tagged site , y chromosome , polymerase chain reaction , azoospermia factor , multiplex polymerase chain reaction , genomic dna , genetics , biology , male infertility , chromosome , medicine , microbiology and biotechnology , computational biology , gene , infertility , pregnancy , gene mapping
Y chromosome microdeletions at the "Azoospermia Factor" regions (AZFa, AZFb, AZFc) are the second genetic cause of spermatogenic failure in infertile men. Despite its importance for the treatment of infertile patients, no prior investigations have been previously published in Ecuador. . The purpose of this study is to optimize a molecular technique that allows detection of microdeletions in the AZF region.