Open AccessComparative analysis of two cryopreservation systems of ovarian tissues in female Wistar rats
Author(s) -
Isabel C. L. O. Durli,
Ana Helena R. Paz,
Paula Barros Terraciano,
Eduardo Pandolfi Passos,
Elizabeth Obino Cirne-Lima
Publication year - 2014
Publication title -
jbra
Language(s) - English
Resource type - Journals
eISSN - 1518-0557
pISSN - 1517-5693
DOI - 10.5935/1518-0557.20140085
Subject(s) - cryopreservation , cryoprotectant , folliculogenesis , andrology , ovary , antral follicle , dimethyl sulfoxide , follicular phase , vacuolization , biology , eosin , oocyte , oogenesis , staining , chemistry , endocrinology , embryo , medicine , microbiology and biotechnology , genetics , organic chemistry
The aim of this study was to determine the most efficient protocol for cryopreservation of ovarian tissue using the automatic Freeze Control® system and to test two different cooling curves combined with two different cryoprotectants: dimethyl sulfoxide (DMSO) and ethylene glycol (EG).