Open AccessNr4A1 mRNA regulation of FSH-induced meiotic resumption in bovine cumulus-oocyte complexes
Author(s) -
Jessica E. Hicks,
Robert M. Petters,
Jeffrey Sommer,
Charlotte E. Farin
Publication year - 2014
Publication title -
jbra
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 14
eISSN - 1518-0557
pISSN - 1517-5693
DOI - 10.5935/1518-0557.20140010
Subject(s) - oocyte , germinal vesicle , messenger rna , gene expression , andrology , biology , transcription (linguistics) , microbiology and biotechnology , chemistry , gene , medicine , genetics , embryo , linguistics , philosophy
1) further define the time course required for transcription initiation in bovine cumulus-oocyte-complexes (COC); 2) determine the pattern of expression for Nr4A1 and Egr1 mRNAs in bovine COC; and, 3) reduce Nr4A1 mRNA expression using small interfering RNAs (siRNA) to determine the effect on breakdown of the germinal vesicle (GVBD).