Open AccessPARTITIONING OF WHEY PROTEINS USING AQUEOUS TWO-PHASE SYSTEMS WITH IONIC LIQUIDS
Author(s) -
Benoît Y. Michel,
Mateus Tomaz Neves,
Rita de Cássia Superbi de Sousa,
Marcelo Martins das Chagas,
Bruna Amaral Martins,
Jane Sélia dos Reis Coimbra
Publication year - 2015
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.5935/0100-4042.20150123
Subject(s) - ionic liquid , partition coefficient , c4mim , lactoferrin , chemistry , chromatography , aqueous solution , lactalbumin , salt (chemistry) , phase (matter) , whey protein , extraction (chemistry) , aqueous two phase system , beta lactoglobulin , biochemistry , organic chemistry , catalysis
In this work, we used an aqueous two-phase system (ATPS) consisting of the ionic liquid [C4mim][Cl] and the salt K2HPO4 to partition α-lactalbumin, β-lactoglobulin, and lactoferrin whey proteins. Extraction efficiency values above 87% indicate that the proteins primarily migrated to the ionic liquid-rich phase (top phase). Partition coefficient values ranged from 6.17 to 8.93 for a-lactalbumin, from 22.80 to 34.55 for β-lactoglobulin, and from 26.46 to 40.06 for lactoferrin. Therefore, the saline ATPS with the ionic liquid examined in this study can be considered to be a promising alternative for extracting whey proteins