Open AccessCHEMICAL CONSTITUENTS, ANTI-INFLAMMATORY, AND FREE-RADICAL SCAVENGING ACTIVITIES OFGuettarda viburnoidesCHAM. & SCHLTDL. (RUBIACEAE)
Author(s) -
Maria Augusta Naressi,
Daniele Domingos Manholer,
Franciele Queiroz Ames,
Ciomar Aparecida Bersani-Amado,
Anelise Samara Nazari Formagio,
Zefa Valdivina Pereira,
Willian Ferreira da Costa,
Débora Cristina Baldoqui,
María Helena Sarragiotto
Publication year - 2015
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.5935/0100-4042.20150093
Subject(s) - rubiaceae , scavenging , botany , traditional medicine , chemistry , horticulture , biology , antioxidant , medicine , organic chemistry
Chemical investigation of Guettarda viburnoides (leaves) led to the isolation of ursolic acid, uncaric acid, secoxyloganin, and grandifloroside, along with a mixture of quercetin-3-O-β-D-galactopyranoside and quercetin-3-O-β-D-glucopyranoside, and of β-sitosterol and stigmasterol. The structures of the isolated compounds were elucidated on the basis of their NMR data. The crude extract, ethyl acetate fraction, aqueous-methanol fraction, and grandifloroside showed significant DPPH free-radical scavenging activities with IC50 ranging from 18.92 to 26.47 µg mL-1. The topical administration of the crude extract and fractions markedly reduced the croton oil-induced mice ear edema in 67.0%-99.0%. Inhibition of tissue MPO activity was also observed, which demonstrated an anti-inflammatory effect of the G. viburnoides species