Open AccessSTATE OF THE ART TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY: FUNDAMENTAL CONCEPTS, INSTRUMENTATION, AND APPLICATIONS
Author(s) -
Noroska Gabriela Salazar Mogollón,
Paula Feliciano de Lima,
Mariana R. Gama,
Mayra Fontes Furlan,
Soraia Cristina Gonzaga Neves Braga,
Paloma Santana Prata,
Isabel Cristina Sales Fontes Jardim,
Fábio Augusto
Publication year - 2014
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.5935/0100-4042.20140261
Subject(s) - instrumentation (computer programming) , state (computer science) , chromatography , computer science , chemistry , algorithm , programming language
The constant evolution of science and the growing demand for new technologies have led to new techniques in instrumentation that can improve detection, separation, resolution, and peak capacity. Comprehensive two-dimensional liquid chromatography (LC×LC) is presented as a powerful tool in complex sample analyses. During an analysis, a sample is subjected to two independent separation mechanisms that are combined, resulting in increased resolving power. For appropriate application of LC×LC, understanding the influence of parameters that require optimization is necessary. The main purpose of optimization is to predict the combination of stationary phases, separation conditions, and instrumental requirements to obtain the best separation performance. This review discusses theoretical, intrumental, and chemometric aspects of LC×LC and focuses on its applications in foods. It aims to provide a clear understanding of the aspects that can be used as strategies in the optimization of this analytical method