Open AccessSIMULTANEOUS DETERMINATION OF CARBAMAZEPINE, PHENYTOIN AND PHENOBARBITAL IN DRIED BLOOD SPOTS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Author(s) -
Gabriela Martins Silva de Lima,
Roberta Zilles Hahn,
Cristina Rama,
Liliane Rhoden,
Paulina Hidalgo,
Cléber Álvares da Silva,
Marina Venzon Antunes,
Rafael Linden
Publication year - 2014
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.5935/0100-4042.20140166
Subject(s) - chromatography , carbamazepine , chemistry , phenobarbital , analyte , phenytoin , spots , dried blood spot , high performance liquid chromatography , extraction (chemistry) , anticonvulsant , pharmacology , medicine , epilepsy , psychiatry
Carbamazepine, phenobarbital and phenytoin were determined in dried blood spots (DBS) by high performance liquid chromatography, after extraction of 8 mm DBS using a mixture of acetonitrile and methanol. Analytes were separated by reversed-phase chromatography, with a run time of 17 minutes. Intra-assay and inter-assay precisions were in the 5.3 to 8.4% and 3.3 to 5.2% ranges, respectively. Accuracy was in the 98.8 to 104.3% range. The method had sensitivity to detect all analytes at levels below minimum therapeutic concentrations. The analytes were stable at 4 ºC and room temperature for up to 12 days and at 45 ºC for 9 days. The method was applied to 14 paired clinical samples of blood serum and DBS