Open AccessKinetic and characterization of rhamnolipid produced byPseudomonas aeruginosaMSIC02 using glycerol as carbon source
Author(s) -
Jorge Rodrigues de Sousa,
Jessyca Aline Costa Correia,
Vânia Maria Maciel Melo,
Leandra Regina Gonçalves,
Antonio José Gonçalves Cruz
Publication year - 2014
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.5935/0100-4042.20140064
Subject(s) - rhamnolipid , glycerol , pulmonary surfactant , surface tension , chemistry , carbon fibers , kinetics , nuclear chemistry , chromatography , materials science , organic chemistry , pseudomonas aeruginosa , biochemistry , thermodynamics , physics , biology , bacteria , genetics , composite material , composite number , quantum mechanics
Glycerol, a co-product of biodiesel production, was used as a carbon source for the kinetics studies and production of biosurfactants by P. aeruginosa MSIC02. The highest fermentative parameters (Y PX = 3.04 g g-1; Y PS = 0.189 g g-1, P B = 31.94 mg L-1 h-1 and P X = 10.5 mg L-1 h-1) were obtained at concentrations of 0.4% (w/v) NaNO3 and 2% (w/v) glycerol. The rhamnolipid exhibited 80% of emulsification on kerosene, surface tension of 32.5 mN m-1, CMC = 28.2 mg L-1, C20 (concentration of surfactant in the bulk phase that produces a reduction of 20 dyn/cm in the surface tension of the solvent) = 0.99 mg L-1, Γm (surface concentration excess) = 2.4 x 10-26 mol Å-2 and S (surface area) = 70.4 Ų molecule-1 with solutions containing 10% NaCl. A mathematical model based on logistic equation was considered to representing the process. Model parameters were estimated by non-linear regression method. This approach was able to give a good description of the process