Open AccessStability-indicating RP-HPLC method for simultaneous determination of gatifloxacin and flurbiprofen in binary combination.
Author(s) -
Islam Ullah Khan,
Syed Naeem Razzaq,
Irfana Mariam,
Muhammad Ashfaq,
Syed Saleem Razzaq
Publication year - 2014
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.5935/0100-4042.20140058
Subject(s) - flurbiprofen , gatifloxacin , chromatography , high performance liquid chromatography , chemistry , binary number , mathematics , pharmacology , biology , biochemistry , ciprofloxacin , antibiotics , arithmetic
A stability-indicating RP-HPLC method is presented for determination of gatifloxacin and flurbiprofen in binary combination. Gatifloxacin, flurbiprofen and their degradation products were detected at 254 nm using a BDS Hypersil C8 (250 X 4.6 mm, 5 µm) column and mixture of 20 mM phosphate buffer (pH 3.0) and methanol 30:70 v/v as mobile phase. Response was linear over the range of 15-105 mg mL-1 for gatifloxacin (r² > 0.998) and of 1.5-10.5 mg mL-1 for flurbiprofen (r² > 0.999). The developed method efficiently separated the analytical peaks from degradation products (peak purity index > 0.9999). The method developed can be applied successfully for determination of gatifloxacin and flurbiprofen in human serum, urine, pharmaceutical formulations, and their stability studies