Open AccessNanoparticles of poly(hydroxybutyrate-co-hydroxyvalerate) as support for the immobilization ofCandida antarcticalipase (Fraction B).
Author(s) -
Ilizandra Aparecida Fernandes,
Nádia Ligianara Dewes Nyari,
J. Vladimir Oliveira,
Elisandra Rigo,
Monique Souza,
Luciana Rocha Barros Gonçalves,
Sibele B. C. Pergher,
Débora de Oliveira
Publication year - 2014
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.5935/0100-4042.20140055
Subject(s) - candida antarctica , lipase , fraction (chemistry) , chemistry , nanoparticle , mass fraction , chromatography , chemical engineering , nuclear chemistry , enzyme , organic chemistry , materials science , nanotechnology , engineering
This work evaluates the immobilization of Candida antarctica lipase (Fraction B) using poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanoparticles as support. The effects of immobilization time (30-150 min) and pH (5-10) on lipase loading were evaluated. The stability of the immobilized enzyme towards temperature (40, 60, and 80 ºC), reuse and storage (at 4 ºC) were also determined. Furthermore, to assess its potential application in a system of interest, the immobilized lipase was used as a catalyst in the esterification of geraniol with oleic acid. The results indicated a time of 120 minutes and pH of 7 as optimal for immobilization. A 21 hour exposure of the PHBV-lipase derivative to 60 ºC showed a 33% reduction of the initial activity while storage at 4 ºC led to a residual activity (5% of the original activity). The derivative was used without significant loss of activity for 4 successive cycles. The use of the immobilized lipase as a catalyst in the production of geranyl oleate led to about 88% conversion of the initial reactants to products